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KMID : 1098420070150020095
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Korean Journal of Medicinal Crop Science
2007 Volume.15 No. 2 p.95 ~ p.99
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Thermotolerant Transgenic Ginseng (Panax ginseng C.A. Meyer) by Introducing Isoprene Synthase Gene through Agrobacterium tumefaciens-mediated Transformation
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Kim Ok-Tae
Hyun Dong-Yun
Bang Kyung-Hwan
Jung Su-Jin
Kim Young-Chang
Shin Yoo-Soo
Kim Dong-Hwi
Kim Swon-Won
Seong Nak-Sull
Cha Seon-Woo
Park Hee-Woon
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Abstract
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The cost of conventional cultivation of ginseng (Panax ginseng C.A. Meyer) is very expensive, because shadow condition should be maintained during cultivation periods owing to inherently weak plant for high-temperature. Therefore, application of plant biotechnology may be possible to overcome these difficulties caused by conventional breeding of ginseng. Transgenic plants were produced via Agrobacterium tumefaciens Gv3101, both carrying the binary plasmid pBI121 mLPISO with nptII and Iso (isoprene synthase) gene. Integration of the transgenes into the P. ginseng nuclear genome was confirmed by PCR analysis using nptII primers and Iso primers. RT-PCR result also demonstrated the foreign isoprene synthase gene in three transgenic plant lines (T1, T3, and T5) which was expressed at the transcriptional level. When whole plants of transgenic ginseng were exposed to high temperature at for 1 h, a non-transformed plant was wilted from heat shock, whereas a transgenic plant appeared to remain healthy. We suggest that the introduction of exogenous isoprene synthase is considered as alternative methods far generating thermotolerance ginseng.
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KEYWORD
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Agrobacterium tumefaciens, Panax ginseng, isoprene synthase, thermotolerance
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